Getting Tested for Celiac Disease

Accurately diagnosing celiac disease can be quite difficult largely because the symptoms often mimic those of other diseases, including irritable bowel syndrome (IBS), Crohn's disease, intestinal infections, lactose intolerance and depression.

Celiac Disease Blood Test | The Gluten Challenge | Endoscopy | Genetic Testing

Step One in Celiac Disease Diagnosis: Blood Test 

Blood tests are the first step in a diagnosis of celiac disease. A doctor will order one or more of a series of blood tests to measure your body's response to gluten.

 

Currently, recommended tests include: 

  • Total IgA
  • IgA-tTG
  • IgA-EMA
  • If IgA is deficient, it is recommended that the IgG/IgA-DGP also be ordered. At the discretion of the doctor, IgG-AGA can also be ordered.

Remain On a Normal, Gluten-Containing Diet Prior to Testing 

Do not change to the gluten-free diet before being tested for celiac disease.  It is important to continue eating a normal, gluten-containing diet before being tested for celiac disease. If the blood tests and symptoms indicate celiac disease, a physician will likely suggest a biopsy of the lining of the small intestine to confirm the diagnosis. See below for more information on the biopsy.

Keep in mind that going gluten-free before being tested for celiac disease can prevent diagnosis.  However, people who have already adopted the gluten-free diet without having been tested for celiac disease can undergo a "gluten challenge" in order to receive accurate test results.

The Gluten Challenge 
 

The gluten challenge now requires eating less gluten over a shorter time frame than in years past. When beginning a gluten challenge:

  • You will be asked to begin to eat gluten again. The gluten challenge will last up to eight weeks
  • You will need to eat a minimum of two servings of gluten (about four slices of bread) per day

While the gluten challenge may sound risky, it is accepted within the medical community. However, you should be closely monitored by a doctor skilled in celiac disease. if taking on the challenge. If you have specific concerns, it is always best to ask your doctor for further guidance.


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Five Reasons You Need to be Tested for Celiac Disease & the Gluten Challenge

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Best Practices in Celiac Disease Diagnosis
 

Q&A iconQ&A: Going Gluten-Free Before the Diagnosis: Do or Don't? with Beyond Celiac Scientific/Medical Advisory Council Member Dr. Joseph A. Murray of the Mayo Clinic 

 

Step Two in Celiac Disease Diagnosis: Endoscopy 

To confirm a celiac disease diagnosis, your doctor may recommend an upper gastrointestinal endoscopy. This procedure will allow your doctor to identify any inflammation or damage in your small intestines, which is a sure sign of celiac disease. Your doctor will take a small biopsy of your intestines during the endoscopy, which will help to confirm your diagnosis. In order for the endoscopy to be accurate, the patient must be on a gluten-containing diet.

An endoscopy may sound like a big procedure, but it only takes about 15 minutes and is a low-risk procedure.

Why It’s Recommended:

While blood tests are helpful in diagnosing celiac disease, they aren’t perfect.  False negatives and false positives are possible, so an endoscopy is needed.   

In the small intestine, there are fingerlike projections called “villi” that help you absorb nutrients. In individuals with celiac disease, gluten damages the villi and causes them to flatten. As a result, the body can’t get the nutrients it needs, which leads to many of the health problems associated with celiac disease. With a biopsy, doctors can see if the villi are flattened.

Endoscopy has long been known as the “gold standard” for celiac disease diagnosis. As blood testing improves, endoscopy may be needed less and less, but for now it remains essential.

Preparation

The actual procedure does not take long, but you will need to take some time to properly prepare. Your doctor will give you a full set of instructions to follow in preparation for the endoscopy, and certain instructions will be dependent upon your age. Here are some brief details of what you can expect.

You will be asked to fast (no food or drink) for 6-8 hours before your endoscopy. This will ensure that your stomach is empty for the procedure. You may also be asked to stop taking certain medications, such as anti-clotting drugs, for a certain period of time before the procedure.

Before the endoscopy begins, you will be sedated and the amount and type of sedation will depend on your age and any other co-existing medical conditions. Sometimes you will be given an anesthetic to numb your throat in addition to the sedative. You will be asked to lie on your back or on your side for the procedure.

During the Endoscopy

During the endoscopy, your doctor will snake a very thin tube through your mouth. When the tube reaches your small intestine, a small tool will be used to take four to eight biopsies from your intestinal wall. After the procedure, a pathologist will use a microscope to examine the extent of damage to the villi. If your villi appear flattened or damaged, it is likely that celiac disease is the cause.

After the Endoscopy

Following the procedure, you will be transferred to a recovery room until any medication or sedative wears off. You may have a mild sore throat or feel slightly bloated as a result of the procedure, but those sensations usually clear up quickly. Most individuals are able to resume eating food within a few hours, after they are fully recovered.

Endoscopy Results

Often, doctors are able to view the image results of an endoscopy that day. But in the case of celiac disease, it’s the actual biopsy results that your physician is most interested in. Therefore, you may be asked to schedule an appointment to go over the results at a later date since it may take a few days for the pathology department to return the biopsy results to your physician.

Positive Test Result:
 

If you test positive for celiac disease, you will need to manage a strict gluten-free diet. Learn more about managing the gluten-free diet here

Negative Test Result: 
 

If your small intestinal biopsy results indicate that celiac disease is not present, but you and your doctor still suspect gluten is the cause of your symptoms, you may want to explore the possibility of non-celiac gluten sensitivity ('gluten sensitivity'). Learn more about gluten sensitivity here

Potential celiac disease is also an option. This is a term used when an individual has a positive celiac disease blood test but a normal small intestinal biopsy. People with potential celiac disease are at an increased risk for developing celiac disease as indicated by positive celiac disease blood tests. This means that they may transition into developing active celiac disease, which is marked by intestinal damage.

At this time, there are no universal guidelines on treating potential celiac disease with a gluten-free diet and most physicians recommend continuing with a regular gluten-containing diet.

Because active celiac disease may develop over time, it is important for individuals with potential celiac disease to monitor any changes in their health such as new signs or symptoms that may indicate celiac disease.

Talk to your doctor about other reasons for your negative biopsy test results and what, if any, future follow-up tests are recommended for you.

Additional information on potential celiac disease can be found here.

What some celiac disease experts are saying about potential celiac disease:

"If the celiac disease blood test is abnormal but their biopsy is negative, I will place a child who has growth failure and is close to puberty on a gluten-free diet and will then have the child undergo a gluten challenge after his or her growth has occurred. This cuts down on the possibility of a child's growth being stunted for life. This practice is relatively common in pediatrics where a child's growth is so critical, and if you miss the window of opportunity prior to puberty you lose height for life."

- Dr. Ritu Verma, Director of the Center for Celiac Disease at The Children's Hospital of Philadelphia and member of NFCA’s Scientific/Medical Advisory Council

“The threshold for treatment in childhood should be lower than for adults with the understanding that this group of biopsy negative symptomatic children should probably carry an indeterminate diagnosis and be challenged in early adulthood.”

- Dr. Dan Leffler, Director of Clinical Research at the Celiac Center at BIDMC and member of NFCA’s Scientific/Medical Advisory Council

Genetic Testing for Celiac Disease 

In order to develop celiac disease, you must have either the HLA-DQ2 or HLA-DQ8 genes. Without one of these genes, it is virtually impossible to develop celiac disease. 

The celiac disease genes alone are not enough to develop celiac disease. Up to 40% of people have these genes, yet only a very small percentage of these people go on to develop it. That's because an environmental trigger is necessary. This trigger can be a stressful event, illness, surgery, pregnancy, etc. Because having the genes does not automatically lead to celiac disease, genetic testing can only rule out celiac disease. It cannot concretely diagnose celiac disease. 

Genetic testing is beneficial for relatives of people diagnosed with celiac disease. Since it is a genetic (hereditary) autoimmune disease, family members are also at risk for developing it. It's recommended that relatives are given the blood test to determine if they have celiac disease. If the test is negative, it should be repeated every 2-3 years or sooner if symptoms occur. That's because celiac disease can develop at any time. Those with negative test results can go on to get the gene test. If the gene test is negative, the relative can stop with regularing screening. 

To learn more about celiac disease in families, visit www.BeyondCeliac.org/TalkTellTest.

If you would like your doctor to have more information about celiac disease, share this free activity with them.

 

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DO YOU THINK YOU MAY HAVE CELIAC DISEASE?

Complete our Celiac Disease Symptoms Checklist today to find out if you could have celiac disease and how to talk to your doctor about getting tested.